Nipah Virus Nucleic Acid Detection Solution

Nipah virus (NiV) is a zoonotic RNA virus belonging to the genus Henipavirus within the family Paramyxoviridae. First identified in Malaysia in 1998, it gained global attention due to outbreaks characterized by encephalitis and severe respiratory symptoms. The natural reservoir is primarily fruit bats (Pteropodidae), with transmission occurring through direct contact with infected animals/their bodily fluids, consumption of contaminated food (e.g., date palm sap), and close human-to-human contact.

 

Human infection with Nipah virus (NiV) manifests with diverse clinical presentations, ranging from asymptomatic infection and acute respiratory syndrome to fatal encephalitis, with a case fatality rate of 40%-75%. Currently, there are no approved antiviral drugs or licensed vaccines specifically targeting NiV. Consequently, early case identification, patient isolation, and interruption of transmission chains constitute critical measures for outbreak control .

 

To address the urgent need for rapid diagnosis of Nipah virus (NiV) infection, BGI Gibel has developed a Nipah Virus Nucleic Acid Detection Kit (PCR-Fluorescent Probe Method) based on its established nucleic acid technology platform. This kit enables highly specific amplification and detection of targeted genomic regions of the virus, featuring operational simplicity, high sensitivity, and short turnaround time. In 2020, it participated in the calibration of reference materials for emerging acute infectious disease virus nucleic acid detection (including NiV) organized by the National Institutes for Food and Drug Control of China. The kit provides stable and reliable support for clinical and public health laboratories, facilitating early screening and confirmation of NiV infection to secure critical time for epidemic containment.


Nipah Virus Nucleic Acid Detection Solution
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Product name

Nipah Virus Nucleic Acid Detection Kit 

                                (PCR-Fluorescent Probe Method)

Product specifications

32 tests/kit

Storage Conditions and Validity Period

This kit must be stored at -18°C protected from light with a 12-month shelf life

Sample Requirements

 Throat swab samples, etc.

Limit of Detection (LoD)

200 Copies/mL

Compatible Instruments

Compatible instruments include the ABI 7500 Real-Time PCR System, Hongshi SLAN-96P Automated Medical PCR Analyzer, Bioer FQD-96A Fluorescence Quantitative PCR System, and Bio-Rad CFX96 Real-Time PCR System, with technical support available for other models.


Technical Advantages

Ultra-Sensitive RT-qPCR Technology:

       ü Utilizes multiplex fluorescent probe design, achieving breakthrough detection sensitivity of 200 copies/mL for Nipah virus.

 Ready-to-Use Master Mix:

ü Single-tube reaction system incorporating human reference primers/probes.

ü Eliminates complex preparation procedures and reduces human error.

 Extensive Instrument Compatibility:

ü Compatible with mainstream international PCR platforms (e.g., ABI, Bioer, Hongshi real-time PCR systems).

ü Enables seamless integration with existing laboratory infrastructure.

 

Application Scenarios

 Port Quarantine Applications:

ü Suitable for international ports (airports/seaports) to conduct rapid screening of travelers and vehicles from epidemic regions.

ü Facilitates early identification and cross-border transmission containment.

 CDC Surveillance Applications:

ü Conduct rapid diagnosis of suspected cases.

ü Implement routine surveillance in high-risk areas (e.g., fruit bat habitats, pig farms) and key populations (close contacts, healthcare workers).

ü Delivers precise detection data for epidemic origin tracing and viral variant surveillance.


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